Born in the Dutch town of Wassenaar in 1965, Anna de Vey Mestdagh studied at the Sweelinck Conservatory in Amsterdam with Bouw Lemkes and Ilja Grubert from 1983 to 1990. Before joining the Royal Concertgebouw Orchestra in 1992, she worked as a substitute with various ensembles and was a member of the Netherlands Radio Philharmonic Orchestra for a short time. She has also played in the Ebony Band.
In addition to playing in the orchestra, de Vey Mestdagh enjoys playing chamber music, an activity she also engages in at home with her husband - bassoonist Gustavo Núñez - and their five children. Writing is also a passion. Since September 2010 she writes columns for Preludium, the Concertgebouw's and Concertgebouw Orchestra's programme magazine.
Since 2014 Anna Veij Mestdagh plays a violin built by Alessandro d'Espine (Turin, 1817), which was purchased by RCO Foundation for former RCO violinist Janke Tamminga, who played this instrument until her retirement in 2014.